IBA offers a full complement of protein purification services. IBA’s expertise allows us to independently develop methods internally yet offer the flexability to employ a procedure that is designed by you. Our goal is to optimize your protein production through classic protein purification methods or new and innovative strategies for those challenging ligands. Protein purification is a stand alone service or can be employed to complement our assay method development, cell-based assay or antibody production services. As a value-added service endotoxin determination and removal as well as stable formulation is available.
Purification Methods:
Salt fractionation
Column chromatography
-gel filtration
-ion exchange
-hydrophobicity
-size exclusion
Affinity chromatography
-Ag or AB based
-protein A / G
-His-tag
Differential centrifugation
Preparative electrophoresis
High-pressure Liquid Chromatography (HPLC)
Reverse-phase Liquid Chromatography (RPLC)
Characterization Analysis:
Denaturing electrophoresis (SDS-PAGE)
Non-denaturing electrophoresis
Western Blot
ELISA
Maldi-TOF
N-terminal amino acid sequencing
LC-MS/MS analysis of tryptic digests
Carbohydrate Analysis (glycation)
Examples of Supported Sample Media
Tissue culture supernatant
Cell extract or homogenates
Tissue extracts or homogenates
Biological fluids (serum, plasma, urine, CSF, saliva, BALF)
To contact an IBA representative call (203) 315-5972 or e-mail: info@iba-analytics.com